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anti nectin2 neutralizing antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti nectin2 neutralizing antibody
    a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – <t>NECTIN2</t> axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.
    Anti Nectin2 Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nectin2 neutralizing antibody/product/R&D Systems
    Average 93 stars, based on 11 article reviews
    anti nectin2 neutralizing antibody - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma"

    Article Title: Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma

    Journal: Nature Communications

    doi: 10.1038/s41467-021-24010-1

    a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – NECTIN2 axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.
    Figure Legend Snippet: a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – NECTIN2 axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.

    Techniques Used: Expressing

    a CellTrace Violet (CTV)-labeled mouse splenic T cells were isolated and co-cultured with Hepa1–6 cells in the presence or absence of anti-Nectin2 neutralizing antibody (15 µg/mL). Mean ± SD is presented. b CTV-labeled T cells were cocultured with Hepa1–6 (WT), - Nectin2 -KO1, - Nectin2 -KO2, - Nectin2 -KO3 cells. Mean ± SD is presented. c Representative picture and weight of Nectin2 WT ( Nectin2 WT: Tp53 KO: c-Myc OE), and Nectin2 KO ( Nectin2 KO: Tp53 KO: c-Myc OE) HCC tumors. Scale bar = 1 cm. d – f Numbers of tumor-infiltrating lymphocytes were analyzed by flow cytometry. g , h Representative pictures, and quantification of CD4 + T cells and CD8 + T cells in HCC tumors by IHC staining. Scale bar = 100μm in IHC representative pictures. a – h Student’s t test. The experiment was performed with a variable number of biologically independent samples ( n number) ( a n = 6, n = 3, and n = 3 for T cells only Ctrl and anti-Nectin2 respectively in CD4+ cells, and n = 3 for all groups in CD8+ cells; b n = 10, n = 6, n = 4, and n = 4 for WT, Nectin2 -KO1, Nectin2 -KO2, and Nectin2 -KO3, respectively in both CD4+ and CD8+ cells; c – e , g : n = 7 per group; f , h : n = 21 per group). Source data are provided as a Source Data file.
    Figure Legend Snippet: a CellTrace Violet (CTV)-labeled mouse splenic T cells were isolated and co-cultured with Hepa1–6 cells in the presence or absence of anti-Nectin2 neutralizing antibody (15 µg/mL). Mean ± SD is presented. b CTV-labeled T cells were cocultured with Hepa1–6 (WT), - Nectin2 -KO1, - Nectin2 -KO2, - Nectin2 -KO3 cells. Mean ± SD is presented. c Representative picture and weight of Nectin2 WT ( Nectin2 WT: Tp53 KO: c-Myc OE), and Nectin2 KO ( Nectin2 KO: Tp53 KO: c-Myc OE) HCC tumors. Scale bar = 1 cm. d – f Numbers of tumor-infiltrating lymphocytes were analyzed by flow cytometry. g , h Representative pictures, and quantification of CD4 + T cells and CD8 + T cells in HCC tumors by IHC staining. Scale bar = 100μm in IHC representative pictures. a – h Student’s t test. The experiment was performed with a variable number of biologically independent samples ( n number) ( a n = 6, n = 3, and n = 3 for T cells only Ctrl and anti-Nectin2 respectively in CD4+ cells, and n = 3 for all groups in CD8+ cells; b n = 10, n = 6, n = 4, and n = 4 for WT, Nectin2 -KO1, Nectin2 -KO2, and Nectin2 -KO3, respectively in both CD4+ and CD8+ cells; c – e , g : n = 7 per group; f , h : n = 21 per group). Source data are provided as a Source Data file.

    Techniques Used: Labeling, Isolation, Cell Culture, Flow Cytometry, Immunohistochemistry



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    anti nectin2 neutralizing antibody  (R&D Systems)
    93
    R&D Systems anti nectin2 neutralizing antibody
    a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – <t>NECTIN2</t> axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.
    Anti Nectin2 Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nectin2 neutralizing antibody/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    anti nectin2 neutralizing antibody - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – NECTIN2 axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma

    doi: 10.1038/s41467-021-24010-1

    Figure Lengend Snippet: a We examined the immune checkpoint interactions between lymphocytes and APCs (tumor cells and TAMs) and identified the prominent interaction via the TIGIT – NECTIN2 axis (circle size indicates the statistical significance and circle color indicates the level of interaction). The empirical P value was estimated by 1000 imputations. b The expression of TIGIT and NECTIN2 was respectively enriched in T cells and APCs. c Upregulation of NECTIN2 was detected in HCC tumors, as compared to non-tumorous livers in both in-house and TCGA datasets. Student’s t test (2-sided). Source data are provided as a Source Data file.

    Article Snippet: In the coculturing experiment of T and parental Hepa1–6 cells, 15 μg/mL anti-Nectin2 neutralizing antibody (MAB3869, R&D Systems, MN, USA) (Supplementary Table ) was added.

    Techniques: Expressing

    a CellTrace Violet (CTV)-labeled mouse splenic T cells were isolated and co-cultured with Hepa1–6 cells in the presence or absence of anti-Nectin2 neutralizing antibody (15 µg/mL). Mean ± SD is presented. b CTV-labeled T cells were cocultured with Hepa1–6 (WT), - Nectin2 -KO1, - Nectin2 -KO2, - Nectin2 -KO3 cells. Mean ± SD is presented. c Representative picture and weight of Nectin2 WT ( Nectin2 WT: Tp53 KO: c-Myc OE), and Nectin2 KO ( Nectin2 KO: Tp53 KO: c-Myc OE) HCC tumors. Scale bar = 1 cm. d – f Numbers of tumor-infiltrating lymphocytes were analyzed by flow cytometry. g , h Representative pictures, and quantification of CD4 + T cells and CD8 + T cells in HCC tumors by IHC staining. Scale bar = 100μm in IHC representative pictures. a – h Student’s t test. The experiment was performed with a variable number of biologically independent samples ( n number) ( a n = 6, n = 3, and n = 3 for T cells only Ctrl and anti-Nectin2 respectively in CD4+ cells, and n = 3 for all groups in CD8+ cells; b n = 10, n = 6, n = 4, and n = 4 for WT, Nectin2 -KO1, Nectin2 -KO2, and Nectin2 -KO3, respectively in both CD4+ and CD8+ cells; c – e , g : n = 7 per group; f , h : n = 21 per group). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma

    doi: 10.1038/s41467-021-24010-1

    Figure Lengend Snippet: a CellTrace Violet (CTV)-labeled mouse splenic T cells were isolated and co-cultured with Hepa1–6 cells in the presence or absence of anti-Nectin2 neutralizing antibody (15 µg/mL). Mean ± SD is presented. b CTV-labeled T cells were cocultured with Hepa1–6 (WT), - Nectin2 -KO1, - Nectin2 -KO2, - Nectin2 -KO3 cells. Mean ± SD is presented. c Representative picture and weight of Nectin2 WT ( Nectin2 WT: Tp53 KO: c-Myc OE), and Nectin2 KO ( Nectin2 KO: Tp53 KO: c-Myc OE) HCC tumors. Scale bar = 1 cm. d – f Numbers of tumor-infiltrating lymphocytes were analyzed by flow cytometry. g , h Representative pictures, and quantification of CD4 + T cells and CD8 + T cells in HCC tumors by IHC staining. Scale bar = 100μm in IHC representative pictures. a – h Student’s t test. The experiment was performed with a variable number of biologically independent samples ( n number) ( a n = 6, n = 3, and n = 3 for T cells only Ctrl and anti-Nectin2 respectively in CD4+ cells, and n = 3 for all groups in CD8+ cells; b n = 10, n = 6, n = 4, and n = 4 for WT, Nectin2 -KO1, Nectin2 -KO2, and Nectin2 -KO3, respectively in both CD4+ and CD8+ cells; c – e , g : n = 7 per group; f , h : n = 21 per group). Source data are provided as a Source Data file.

    Article Snippet: In the coculturing experiment of T and parental Hepa1–6 cells, 15 μg/mL anti-Nectin2 neutralizing antibody (MAB3869, R&D Systems, MN, USA) (Supplementary Table ) was added.

    Techniques: Labeling, Isolation, Cell Culture, Flow Cytometry, Immunohistochemistry